Ubiquitin E3 ligase A20 facilitates processing microbial product in nasal epithelial cells

J Biol Chem. 2012 Oct 12;287(42):35318-35323. doi: 10.1074/jbc.M112.392639. Epub 2012 Aug 30.

Abstract

Microbial products play a role in the pathogenesis of allergic diseases; ubiquitin E3 ligase A20 (A20) is an important molecule in regulating inflammation in the body. The present study aims to elucidate the role of A20 in processing the absorbed microbial products in nasal epithelial cells. Human nasal mucosal specimens were collected from patients with or without chronic rhinitis and analyzed by immunohistochemistry. Human nasal epithelial cell line, RPMI2650 cell, was employed to assess the role of A20 in processing the absorbed staphylococcal enterotoxin B (SEB). The RPMI2650 cells absorbed SEB in the culture. The increase in A20 was observed in RPMI2650 cells in parallel to the absorption of SEB. A20 is a critical molecule in the degradation of SEB in the nasal epithelial cells by promoting the tethering of endosomes and lysosomes. A20 plays a critical role in processing of the absorbed SEB in nasal epithelial cells.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Bacterial Toxins / genetics
  • Bacterial Toxins / metabolism*
  • Cell Line
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Endosomes / genetics
  • Endosomes / metabolism
  • Enterotoxins / metabolism*
  • Epithelial Cells / enzymology*
  • Epithelial Cells / microbiology
  • Female
  • Humans
  • Intracellular Signaling Peptides and Proteins / genetics
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Lysosomes / genetics
  • Lysosomes / metabolism
  • Male
  • Nasal Mucosa / enzymology*
  • Nasal Mucosa / microbiology
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Proteolysis*
  • Staphylococcus aureus / metabolism*
  • Tumor Necrosis Factor alpha-Induced Protein 3
  • Ubiquitin-Protein Ligases / genetics
  • Ubiquitin-Protein Ligases / metabolism*

Substances

  • Bacterial Proteins
  • Bacterial Toxins
  • DNA-Binding Proteins
  • Enterotoxins
  • Intracellular Signaling Peptides and Proteins
  • Nuclear Proteins
  • enterotoxin B, staphylococcal
  • Ubiquitin-Protein Ligases
  • TNFAIP3 protein, human
  • Tumor Necrosis Factor alpha-Induced Protein 3