Abstract
We herein describe a molecular analysis based on the 3' region of the glycoprotein C gene of 45 bovine herpesvirus (BoHV) isolates from Brazil (n=41), Uruguay (n=2) and Argentina (n=2). Nucleotide (nt) sequencing and alignment of 333 nt revealed levels of similarity ranging from 99.1% to 100% among BoHV-1 sequences (n=12); 96.2-100% among BoHV-5 sequences (n=32); and 77.7-90.3% between BoHV-1 and BoHV-5 sequences. The phylogenetic tree reconstruction provided a clear distinction between BoHV-1 and BoHV-5, and BoHV-1 into subtypes BoHV-1.1 and BoHV-1.2. The isolate SV 453/93 (BoHV-1 associated with genital disease) could not be included within BoHV-1 subtypes since it presented a markedly distinct nt and amino acid (aa) deduced sequences. A transmembrane domain of 24 aa and the putative cytoplasmic tail of 8 aa were identified and mapped. These results indicate that this genome region represents a suitable target for phylogenetic subgrouping of BoHV-1 and BoHV-5 isolates and, perhaps, for understanding evolutionary relationships.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Argentina
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Base Sequence
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Brazil
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Cattle / virology
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DNA, Viral / genetics
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Herpesvirus 1, Bovine / genetics*
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Herpesvirus 5, Bovine / genetics*
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Molecular Sequence Data
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Phylogeny
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Sequence Alignment / veterinary
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Sequence Analysis, DNA / veterinary
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Uruguay
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Viral Envelope Proteins / genetics
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Viral Proteins / genetics*
Substances
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DNA, Viral
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Viral Envelope Proteins
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Viral Proteins
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bovine herpesvirus type-1 glycoproteins
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glycoprotein gC, herpes simplex virus type 1
Associated data
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GENBANK/JN173204
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GENBANK/JN173205
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GENBANK/JN173206
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GENBANK/JN173207
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