Abstract
Set2-mediated methylation of histone H3 Lys36 (H3K36) is a mark associated with the coding sequences of actively transcribed genes, but it has a negative role during transcription elongation. It prevents trans-histone exchange over coding regions and signals for histone deacetylation in the wake of RNA polymerase II (RNAPII) passage. We have found that in Saccharomyces cerevisiae the Isw1b chromatin-remodeling complex is specifically recruited to open reading frames (ORFs) by H3K36 methylation through the PWWP domain of its Ioc4 subunit in vivo and in vitro. Isw1b acts in conjunction with Chd1 to regulate chromatin structure by preventing trans-histone exchange from taking place over coding regions. In this way, Isw1b and Chd1 are important in maintaining chromatin integrity during transcription elongation by RNAPII.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Adenosine Triphosphatases / analysis
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Adenosine Triphosphatases / genetics
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Adenosine Triphosphatases / metabolism*
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Chromatin / chemistry
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Chromatin / metabolism*
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DNA-Binding Proteins / analysis
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Deletion
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Gene Expression Regulation, Fungal*
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Histones / analysis
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Histones / metabolism*
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Methyltransferases / metabolism
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Open Reading Frames
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Saccharomyces cerevisiae / chemistry
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / analysis
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Transcription, Genetic
Substances
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CHD1 protein, S cerevisiae
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Chromatin
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DNA-Binding Proteins
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Histones
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IOC4 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Methyltransferases
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Set2 protein, S cerevisiae
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Adenosine Triphosphatases
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ISW1 protein, S cerevisiae