Estimation of paracrine signaling using quantitative RT-PCR from multiple patchy lesion samples

Shuang Jiao; Haruo Hanawa; Tomoyasu Suzuki; Ying Hua Hu; Kaori Yoshida; Minako Ogura; Yukako Ohno; Masahiro Ito; Takeshi Kashimura; Hiroaki Obata; Makoto Kodama

doi:10.1016/j.mcp.2012.08.002

Estimation of paracrine signaling using quantitative RT-PCR from multiple patchy lesion samples

Mol Cell Probes. 2013 Apr;27(2):109-13. doi: 10.1016/j.mcp.2012.08.002. Epub 2012 Aug 17.

Authors

Shuang Jiao¹, Haruo Hanawa, Tomoyasu Suzuki, Ying Hua Hu, Kaori Yoshida, Minako Ogura, Yukako Ohno, Masahiro Ito, Takeshi Kashimura, Hiroaki Obata, Makoto Kodama

Affiliation

¹ Division of Cardiology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.

PMID: 22921513
DOI: 10.1016/j.mcp.2012.08.002

Abstract

We investigated whether correlations between mRNA levels of cytokines versus other proteins from patchy lesion could estimate cytokine paracrine signaling in vivo. Experiments with rat experimental autoimmune myocarditis (EAM), a patchy myocarditis model, indicated IL-1 and other protein levels were correlated, indicating paracrine signaling pathways in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Autoimmune Diseases / metabolism
Autoimmune Diseases / pathology
Disease Models, Animal
Interleukin-1 / genetics
Myocarditis / genetics
Myocarditis / metabolism*
Myocarditis / pathology
Paracrine Communication / genetics*
RNA, Messenger / analysis
Rats
Reverse Transcriptase Polymerase Chain Reaction / methods*

Substances

Interleukin-1
RNA, Messenger