Neuronal recordings with solid-conductor intracellular nanoelectrodes (SCINEs)

PLoS One. 2012;7(8):e43194. doi: 10.1371/journal.pone.0043194. Epub 2012 Aug 15.

Abstract

Direct electrical recording of the neuronal transmembrane potential has been crucial to our understanding of the biophysical mechanisms subserving neuronal computation. Existing intracellular recording techniques, however, limit the accuracy and duration of such measurements by changing intracellular biochemistry and/or by damaging the plasma membrane. Here we demonstrate that nanoengineered electrodes can be used to record neuronal transmembrane potentials in brain tissue without causing these physiological perturbations. Using focused ion beam milling, we have fabricated Solid-Conductor Intracellular NanoElectrodes (SCINEs), from conventional tungsten microelectrodes. SCINEs have tips that are <300 nm in diameter for several micrometers, but can be easily handled and can be inserted into brain tissue. Performing simultaneous whole-cell patch recordings, we show that SCINEs can record action potentials (APs) as well as slower, subthreshold neuronal potentials without altering cellular properties. These results show a key role for nanotechnology in the development of new electrical recording techniques in neuroscience.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / physiology*
  • Animals
  • Biophysics / methods
  • Electric Capacitance
  • Electric Impedance
  • Electrodes
  • Hippocampus / pathology
  • Materials Testing
  • Membrane Potentials
  • Microelectrodes*
  • Nanotechnology / methods*
  • Neurons / metabolism
  • Neurons / physiology*
  • Neurosciences / methods
  • Patch-Clamp Techniques / methods*
  • Rats
  • Silanes / chemistry

Substances

  • Silanes

Grants and funding

Max Planck Society, University of Heidelberg. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.