Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: color-based selection of colonies using the met30 strain

J Biosci Bioeng. 2012 Dec;114(6):619-21. doi: 10.1016/j.jbiosc.2012.07.011. Epub 2012 Aug 11.

Abstract

Glutathione overproducers were detected by examining the pigmentation intensity of Saccharomyces cerevisiae met30 yeast carrying wild-type alleles for ADE1 and ADE2. Highly pigmented colonies, phenocopies of the ade2 or ade1 mutants, were observed among yeast grown in minimal biotin-free medium with a high methionine content.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Cell Separation / methods
  • Color*
  • Culture Media / chemistry
  • Culture Media / pharmacology
  • F-Box Proteins / genetics*
  • Genes, Fungal / genetics*
  • Glutathione / analysis
  • Glutathione / chemistry
  • Glutathione / metabolism
  • Methionine / metabolism
  • Methionine / pharmacology
  • Mutation / genetics*
  • Phenotype
  • Pigmentation / genetics
  • Saccharomyces cerevisiae / chemistry*
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / isolation & purification*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Sulfur / analysis*
  • Ubiquitin-Protein Ligase Complexes / genetics*

Substances

  • Culture Media
  • F-Box Proteins
  • MET30 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sulfur
  • Methionine
  • Ubiquitin-Protein Ligase Complexes
  • Glutathione