Abstract
Glutathione overproducers were detected by examining the pigmentation intensity of Saccharomyces cerevisiae met30 yeast carrying wild-type alleles for ADE1 and ADE2. Highly pigmented colonies, phenocopies of the ade2 or ade1 mutants, were observed among yeast grown in minimal biotin-free medium with a high methionine content.
Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Cell Separation / methods
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Color*
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Culture Media / chemistry
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Culture Media / pharmacology
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F-Box Proteins / genetics*
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Genes, Fungal / genetics*
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Glutathione / analysis
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Glutathione / chemistry
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Glutathione / metabolism
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Methionine / metabolism
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Methionine / pharmacology
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Mutation / genetics*
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Phenotype
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Pigmentation / genetics
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Saccharomyces cerevisiae / chemistry*
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / isolation & purification*
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Saccharomyces cerevisiae Proteins / genetics*
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Sulfur / analysis*
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Ubiquitin-Protein Ligase Complexes / genetics*
Substances
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Culture Media
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F-Box Proteins
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MET30 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Sulfur
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Methionine
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Ubiquitin-Protein Ligase Complexes
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Glutathione