In vitro and in vivo antitumor activity of doxorubicin-loaded alginic-acid-based nanoparticles

Macromol Biosci. 2012 Oct;12(10):1326-35. doi: 10.1002/mabi.201200165. Epub 2012 Aug 7.

Abstract

The antitumor activities of DOX-loaded alginic acid/poly[2-(diethylamino)ethyl methacrylate] (ALG-PDEA) nanoparticles are evaluated both in vitro and in vivo. TEM imaging shows that the ALG-PDEA NPs have a spherical morphology with a size of about 120 nm. CLSM observations reveal that the negatively charged ALG-PDEA NPs can be taken up well by cells. In vivo NIR fluorescence imaging shows that the ALG-PDEA NPs can passively target the tumor area because of the EPR effect in the H22 tumor-bearing mouse. In vivo antitumor efficacy examinations indicate that DOX-loaded ALG-PDEA NPs have significantly superior efficacy in impeding tumor growth compared to free DOX and low toxicity to living mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates*
  • Animals
  • Antibiotics, Antineoplastic / administration & dosage
  • Antibiotics, Antineoplastic / pharmacokinetics
  • Antibiotics, Antineoplastic / pharmacology*
  • Biological Transport
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Doxorubicin / administration & dosage
  • Doxorubicin / pharmacokinetics
  • Doxorubicin / pharmacology*
  • Drug Carriers
  • Drug Compounding
  • Fluorescent Dyes
  • Liver Neoplasms / drug therapy*
  • Liver Neoplasms / pathology
  • Male
  • Mice
  • Mice, Inbred ICR
  • Microscopy, Electron, Transmission
  • Microscopy, Fluorescence
  • Nanoparticles
  • Neoplasm Transplantation
  • Optical Imaging
  • Particle Size
  • Polymethacrylic Acids*

Substances

  • Alginates
  • Antibiotics, Antineoplastic
  • Drug Carriers
  • Fluorescent Dyes
  • Polymethacrylic Acids
  • alginic acid-poly(2-(diethylamino)ethyl methacrylate)
  • Doxorubicin