Microfluidic PCR combined with pyrosequencing for identification of allelic variants with phenotypic associations among targeted Salmonella genes

Appl Environ Microbiol. 2012 Oct;78(20):7480-2. doi: 10.1128/AEM.01703-12. Epub 2012 Aug 10.

Abstract

A novel targeted massive parallel sequencing approach identified genetic variation in eight known or predicted fimbrial adhesins for 46 Salmonella strains. The results highlight associations between specific adhesin alleles, host species, and antimicrobial resistance. The differentiation of allelic variants has potential applications for diagnostic microbiology and epidemiological investigations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Bacterial / genetics*
  • Alleles
  • Anti-Bacterial Agents / pharmacology
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Drug Resistance, Bacterial
  • Genetic Variation*
  • Genotype
  • High-Throughput Nucleotide Sequencing / methods*
  • Host Specificity
  • Microfluidic Analytical Techniques / methods*
  • Molecular Epidemiology / methods
  • Molecular Sequence Data
  • Molecular Typing
  • Phenotype
  • Polymerase Chain Reaction / methods*
  • Salmonella / classification
  • Salmonella / drug effects
  • Salmonella / genetics*
  • Salmonella / physiology

Substances

  • Adhesins, Bacterial
  • Anti-Bacterial Agents
  • DNA, Bacterial

Associated data

  • GDB/JX026805
  • GDB/JX026806
  • GDB/JX026807
  • GDB/JX026808
  • GDB/JX026809
  • GDB/JX026810
  • GDB/JX026811
  • GDB/JX026812
  • GDB/JX026813
  • GDB/JX026814
  • GDB/JX026815
  • GDB/JX026816
  • GDB/JX026817
  • GDB/JX026818
  • GDB/JX026819
  • GDB/JX026820
  • GDB/JX026821
  • GDB/JX026822
  • GDB/JX026823
  • GDB/JX026824
  • GDB/JX026825
  • GDB/JX026826
  • GDB/JX026827
  • GDB/JX026828
  • GDB/JX026829
  • GDB/JX026830
  • GDB/JX026831