Phenolic acids in the flowers and leaves of Grindelia robusta Nutt. and Grindelia squarrosa Dun. (Asteraceae)

Acta Pol Pharm. 2012 Jul-Aug;69(4):693-8.

Abstract

2D-TLC and RP-HPLC methods were applied to qualitatively determinate free phenolic acids and those liberated by acid and alkaline hydrolysis in the flowers and leaves of G. robusta and G. squarrosa. The presence of eleven phenolic acids, namely: caffeic, chlorogenic, p-coumaric, p-hydroxybenzoic, ferulic, gallic, protocatechuic, vanillic salicylic, p-hydroxyphenylacetic and ellagic acids was determined. Quantitative estimate of phenolic acids, expressed as caffeic acid, has been analyzed by the method described in the Polish Pharmacopoeia VIII. The content of phenolic acids in G. robusta reached 7.33 mg/g and 6.23 mg/g for flowers and leaves, respectively. The flowers and leaves of G. squarrosa were characterized by similar level of phenolic acids, namely 6.81 mg/g and 6.59 mg/g, respectively.

MeSH terms

  • Calibration
  • Chromatography, High Pressure Liquid / standards
  • Chromatography, Thin Layer / standards
  • Flowers
  • Grindelia / chemistry*
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Hydroxybenzoates / analysis*
  • Plant Leaves
  • Reference Standards

Substances

  • Hydroxybenzoates
  • phenolic acid