Abstract
Immediate-type hypersensitivity is characterized by elevated levels of immunoglobulin E (IgE) and activated mast cell plays a crucial role by releasing granule contents, lipid-derived mediators, cytokines, and chemokines. To evaluate the antiallergic effects of panduratin A isolated from Boesenbergia pandurata Roxb., we determined its effects on calcium (Ca(2+)) influx, degranulation, and inflammatory mediators in calcium ionophore A23187 and phorbol 12-myristate 13-acetate (PMA)-stimulated rat basophilic leukemia (RBL-2H3) cells. Panduratin A (20 μM) inhibited secretion of β-hexosaminidase (46.69 ± 9.6 %), histamine (34.32 ± 2.1 %), and Ca(2+) influx (43.84 %). Panduratin A reduced the production of prostaglandin E(2) (PGE(2), 47.58 ± 3.4 %), leukotriene B(4) (LTB(4), 98.15 ± 1.6 %), and the mRNA expression of cyclooxygenase-2, 5-lipoxygenase, interleukin (IL)-4, IL-13, and tumor necrosis factor-α. Furthermore, panduarin A attenuated phosphorylation of Akt, the mitogen-activated protein kinases (MAPK) extracellular signal-regulated kinase (ERK), p38, and c-Jun N-terminal kinase (JNK) expression. These results indicate that panduratin A might be useful as an agent against immediate-type hypersensitivity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arachidonate 5-Lipoxygenase / genetics
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Calcimycin / pharmacology
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Calcium / metabolism
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Cell Degranulation / drug effects
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Cell Degranulation / immunology
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Cell Line, Tumor
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Chalcones / pharmacology*
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Cyclooxygenase 2 / genetics
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Extracellular Signal-Regulated MAP Kinases / metabolism
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Histamine / metabolism
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Histamine Release / drug effects*
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Histamine Release / immunology
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Hypersensitivity, Immediate / drug therapy*
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Immunoglobulin E / immunology
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Inflammation Mediators
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Interleukin-13 / genetics
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Interleukin-4 / genetics
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JNK Mitogen-Activated Protein Kinases / metabolism
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Leukemia, Basophilic, Acute / drug therapy*
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Leukotriene B4 / biosynthesis
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Mast Cells* / drug effects
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Mast Cells* / immunology
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Mast Cells* / metabolism
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Phosphorylation
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Plant Extracts / pharmacology
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Prostaglandins E / biosynthesis
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Proto-Oncogene Proteins c-akt / metabolism
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RNA, Messenger / biosynthesis
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Rats
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Signal Transduction / immunology
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Tetradecanoylphorbol Acetate / pharmacology
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Tumor Necrosis Factor-alpha / genetics
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Zingiberaceae / metabolism
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beta-N-Acetylhexosaminidases / drug effects*
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beta-N-Acetylhexosaminidases / metabolism
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p38 Mitogen-Activated Protein Kinases / metabolism
Substances
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Chalcones
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Inflammation Mediators
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Interleukin-13
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Plant Extracts
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Prostaglandins E
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Leukotriene B4
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Interleukin-4
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panduratin A
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Immunoglobulin E
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Calcimycin
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Histamine
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Arachidonate 5-Lipoxygenase
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Cyclooxygenase 2
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Proto-Oncogene Proteins c-akt
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Extracellular Signal-Regulated MAP Kinases
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JNK Mitogen-Activated Protein Kinases
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p38 Mitogen-Activated Protein Kinases
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beta-N-Acetylhexosaminidases
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Tetradecanoylphorbol Acetate
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Calcium