Characterization of new microsatellite loci isolated from Santiria trimera (Burseraceae)

Am J Bot. 2012 Aug;99(8):e334-6. doi: 10.3732/ajb.1200041. Epub 2012 Jul 30.

Abstract

Premise of the study: To study the genetic structure among three morphotypes of an African rainforest tree species, Santiria trimera, nuclear microsatellite markers were isolated and characterized.

Methods and results: Seven polymorphic loci were isolated using a pyrosequencing-based protocol and successfully amplified on three different morphotypes of S. trimera. For six of the seven loci, there is at least one private allele for one of the three morphotypes. The mean effective number of alleles is about four for each of the three morphotypes.

Conclusions: These microsatellite markers are promising to explore the genetic delimitation among sympatric morphotypes found in Gabonese forests and to study the spatial genetic structure within each gene pool.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Base Sequence
  • Burseraceae / classification
  • Burseraceae / genetics*
  • DNA Primers / genetics*
  • DNA, Plant / genetics
  • Genetic Loci
  • Genetic Markers
  • Microsatellite Repeats / genetics*
  • Molecular Sequence Data
  • Polymorphism, Genetic*
  • Sequence Analysis, DNA
  • Species Specificity
  • Trees

Substances

  • DNA Primers
  • DNA, Plant
  • Genetic Markers

Associated data

  • GENBANK/JN258596
  • GENBANK/JN258597
  • GENBANK/JN258598
  • GENBANK/JN258599
  • GENBANK/JN258600
  • GENBANK/JN258601
  • GENBANK/JN258602