Thrombin-stimulated discharge of calcium stores in human platelets: analysis of experimental data

Abstract

The purpose of this research was to analyze experimental data concerning thrombin-stimulated discharge of calcium stores in human platelets contained in calcium-free medium in view of better understanding the mechanisms involved in calcium fluxes. The model curves are reasonably close to experimental data; the parameters of the models are related to the properties of the entities responsible for control or maintenance of cytosolic calcium concentration. It has been shown that: (a) time-course of calcium concentration in cytosol of human platelets can be acceptably modeled on the basis of reasonable assumptions concerning agonist stimulated calcium redistribution in cellular compartments; (b) those assumptions are of fundamental importance for the model (c) some parameters of the model (taken arbitrarily) cannot be estimated independently of others from fitting the model to experimental data available; (d) special experiments are necessary to determine the unknown parameters; (e) agonist-stimulated change of the permeability of endomembrane of calcium stores can be regarded as a pulse of the permeability; it can be modeled as a sequence of transitions of the system from inactive to active and to inactive state again.