Abstract
Bat coronavirus (BtCoV) is assumed to be a progenitor of severe acute respiratory syndrome (SARS)-related coronaviruses. To explore the distribution of BtCoVs in the Philippines, we collected 179 bats and detected viral RNA from intestinal or fecal samples by RT-PCR. The overall prevalence of BtCoVs among bats was 29.6 %. Phylogenetic analysis of the partial RNA-dependent RNA polymerase gene suggested that one of the detected BtCoVs was a novel alphacoronavirus, while the others belonged to the genus Betacoronavirus. Western blotting revealed that 66.5 % of bat sera had antibodies to BtCoV. These surveys suggested the endemic presence of BtCoVs in the Philippines.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / blood
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Base Sequence
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Chiroptera*
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Coronavirus / classification*
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Coronavirus / genetics*
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Coronavirus / immunology
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Coronavirus Infections / epidemiology
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Coronavirus Infections / veterinary*
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Coronavirus Infections / virology
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Coronavirus Nucleocapsid Proteins
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DNA, Complementary / chemistry
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Feces / virology
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Gene Expression Regulation, Viral / physiology
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Intestines / virology
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Molecular Sequence Data
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Nucleocapsid Proteins / genetics
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Nucleocapsid Proteins / metabolism
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Philippines / epidemiology
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Phylogeny
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RNA, Viral / genetics
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RNA, Viral / isolation & purification
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Species Specificity
Substances
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Antibodies, Viral
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Coronavirus Nucleocapsid Proteins
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DNA, Complementary
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Nucleocapsid Proteins
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RNA, Viral
Associated data
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GENBANK/AB683970
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GENBANK/AB683971