Objective: To study the HPLC fingerprint of yunnan Dipsacus asper and provide a reliable method for sciencetific evaluation and quality control.
Methods: 16 batches of Dipsacus asper were collected from 11 counties (cities, districts) of 5 prefectures or municipals in Yunnan provinice, which were the mainly distribution region of Dipsacus asper. Samples were analyzed on an Aglient Zorbax SB-18 (4.6 mm x 250 mm, 5 microm) eluted with methanol (A) and water (B) as mobile phase in gradient clution. The flow rate was 1 ml/min, and the column temperature was set at 38 degrees C. The detector wavelength was 220 nm.
Results: A HPLC fingerprint method was established, 14 common peaks were selected and the similarity ranged from 0.674 to 0.965, cluster analysis could classified these 16 batches of Dipsacus asper into 2 groups.
Conclusion: Dipsacus japonicus is found for the first time in yunnan. The fingerprint of Dipsacus asper in different origin have a extremely high similarity;The method is accurate and reliable.