A quantitative, comprehensive multiresidue method which includes 20 coccidiostat residues has been developed. The method described uses a simple one-step liquid extraction with acetonitrile to isolate analytes from both the polyether ionophore and chemical classes of coccidiostats. Subsequent to a further concentration step, samples were analysed via UHPLC-MS/MS. The method was validated according to the Commission Decision 2002/657/EEC in egg and avian muscle. The method permitted quantitative confirmation for 13 compounds below target concentrations, and screening for a further 7 compounds. Within-laboratory repeatability gave accuracy values in the range of 68-129%, while reproducibility ranged between 75 and 123%. Calibration ranges were typically 1-50 μg kg⁻¹, although higher ranges were used for dinitrocarbanilide, imidocarb and toltrazuril residues. A regression coefficient (R²) value of greater than 0.98 was obtained for all analytes. Precision results ranged from 2.3 to 19.7% CV for egg and from 2.6 to 23.6% CV in muscle. CCα was in the range from 1.13 μg kg⁻¹ (clopidol) to 179 μg kg⁻¹ (lasalocid) in egg. In muscle, CCα ranged from 2.25 μg kg⁻¹ (aprinocid) to 4579 μg kg⁻¹ (dinitrocarbanilide). CCβ was from 1.29 μg kg⁻¹ (clopidol) to 209 μg kg⁻¹ (lasalocid) in egg, and 2.58 μg kg⁻¹ (arprinocid) to 6060 μg kg⁻¹ (dinitrocarbanilide) in muscle. Limits of quantification were 1 μg kg⁻¹ for all compounds, except imidocarb and dinitrocarbanilide (10 μg kg⁻¹), and toltrazuril and metabolites (50 μg kg⁻¹).
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