Cell-penetrating peptides as delivery vehicles for a protein-targeted terbium complex

Chemistry. 2012 Aug 27;18(35):10825-9. doi: 10.1002/chem.201201805. Epub 2012 Jul 17.

Abstract

Release after transmission: Arginine-rich, cell-penetrating peptides (CPPs) mediate cytoplasmic delivery of trimethoprim (TMP)-terbium complex conjugates and selective, intracellular labeling of E. coli dihydrofolate reductase (eDHFR) fusion proteins. A disulfide bond linking CPP and cargo is reduced following uptake. CPP conjugation can be used to deliver otherwise cell-impermeable, ligand-fluorophore conjugates.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arginine / chemistry*
  • Arginine / metabolism
  • Cell Line
  • Cell Membrane Permeability
  • Cell-Penetrating Peptides / chemistry*
  • Cell-Penetrating Peptides / metabolism
  • Dogs
  • Drug Carriers / chemistry*
  • Drug Carriers / metabolism
  • Endocytosis
  • Escherichia coli
  • Fluorescent Dyes
  • Luminescent Proteins / chemistry
  • Luminescent Proteins / metabolism
  • Molecular Imaging
  • Molecular Sequence Data
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Staining and Labeling
  • Terbium / chemistry*

Substances

  • Cell-Penetrating Peptides
  • Drug Carriers
  • Fluorescent Dyes
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Terbium
  • Arginine