Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant

Harold J Schreier; Wipawee Dejtisakdi; Jose Ortega Escalante; Marina Brailo

doi:10.1128/AEM.01794-12

Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant

Appl Environ Microbiol. 2012 Oct;78(19):7120-3. doi: 10.1128/AEM.01794-12. Epub 2012 Jul 13.

Authors

Harold J Schreier¹, Wipawee Dejtisakdi, Jose Ortega Escalante, Marina Brailo

Affiliation

¹ Department of Marine Biotechnology, Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, Maryland, USA. Schreier@umbc.edu

Abstract

A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Culture Media / chemistry
DNA Transposable Elements*
Gluconeogenesis
Glucose / metabolism
Mutagenesis, Insertional / methods*
Phosphoenolpyruvate Carboxykinase (ATP) / deficiency*
Phosphoenolpyruvate Carboxykinase (ATP) / metabolism
Planctomycetales / enzymology*
Planctomycetales / genetics
Planctomycetales / growth & development
Planctomycetales / metabolism

Substances

Culture Media
DNA Transposable Elements
Phosphoenolpyruvate Carboxykinase (ATP)
Glucose