Assessment of lower extremity bilateral asymmetries in soccer players is important for both injury prevention and performance. The purpose of this investigation was to compare isokinetic knee extensor assessment of asymmetry with a more specific countermovement jump (CMJ). Forty-six Brazilian male professional soccer players participated in this study. The maximal power, maximal force and impulse were determined during CMJ and the total work and peak torque at 60, 180, and 300°·s during isokinetic leg extension, separately for each leg. Factor analysis was performed for all investigated variables, and the diagnostic concordance between different criteria was analyzed by McNemar's χ test. The factor analysis showed that the isokinetic and CMJ tests were widely independent methods for the assessment of bilateral differences. Concordance of the diagnostic information could only be found between the maximal force during CMJ and the total work and peak torque at 180 and 300°·s during isokinetic leg extension. Impulse and maximal power during CMJ on a double force platform appear to be appropriate additional variables for the identification of bilateral differences. Therefore, it might be pertinent to perform, in addition to isokinetic assessment, a vertical jump test on a force platform to assure widespread and reliable diagnostic information.