Abstract
A specific technique capable of producing high-quality RNA for rapid amplification of cDNA ends (RACE) was established for challenging tissues: leaves of the rubber tree. Total RNA was extracted by cetyltrimethylammonium bromide (CTAB)-LiCl combined with TRIzol reagent. The isolated RNA was highly intact. With RNA as template, full-length cDNA was obtained (NCBI, AY461413) by RACE.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cetrimonium
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Cetrimonium Compounds / chemistry
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DNA, Complementary / genetics
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Databases, Genetic
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Electrophoresis, Agar Gel
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Guanidines / chemistry
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Hevea / chemistry*
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Hevea / genetics
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Lithium Chloride / chemistry
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Nucleic Acid Amplification Techniques
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Phenols / chemistry
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Plant Leaves / chemistry*
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Plant Leaves / genetics
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RNA, Plant / genetics
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RNA, Plant / isolation & purification*
Substances
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Cetrimonium Compounds
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DNA, Complementary
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Guanidines
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Phenols
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RNA, Plant
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trizol
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Lithium Chloride
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Cetrimonium