Abstract
To investigate the adjuvant effect of granulocyte macrophage colony stimulating factor (GM-CSF) in Flaviviridae virus DNA vaccines. After DNA immunization, the antibody levels of serum from mice were detected by ELISA and indirect immunofluorescence assay. Co-immunization of GM-CSF suppressed the immune responses induced by DV1 and DV2 candidate vaccines whereas enhanced the immune response induced by HCV C and E1 DNA vaccines. As genetic adjuvant for DNA vaccines, GM-CSF might display complex diversity on the immune responses: an augmentation or suppression due to different immunogens. Therefore, GM-CSF should be used with some cautions in clinic.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / administration & dosage*
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Animals
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Antibodies, Viral / immunology
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DNA, Viral / administration & dosage
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DNA, Viral / genetics
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DNA, Viral / immunology*
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Dengue / immunology*
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Dengue / prevention & control
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Dengue / virology
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Dengue Vaccines / administration & dosage
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Dengue Vaccines / genetics
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Dengue Vaccines / immunology
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Dengue Virus / genetics
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Dengue Virus / immunology
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Female
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Granulocyte-Macrophage Colony-Stimulating Factor / administration & dosage
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Granulocyte-Macrophage Colony-Stimulating Factor / immunology*
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Hepacivirus / genetics
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Hepacivirus / immunology
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Hepatitis C / immunology*
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Hepatitis C / prevention & control
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Hepatitis C / virology
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Humans
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Immunization
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Mice
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Mice, Inbred BALB C
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Vaccines, DNA / administration & dosage
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Vaccines, DNA / genetics
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Vaccines, DNA / immunology
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Viral Vaccines / administration & dosage
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Viral Vaccines / genetics
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Viral Vaccines / immunology*
Substances
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Adjuvants, Immunologic
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Antibodies, Viral
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DNA, Viral
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Dengue Vaccines
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Vaccines, DNA
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Viral Vaccines
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Granulocyte-Macrophage Colony-Stimulating Factor