Yiqi Fumai freeze-dried powder for injection was used as a model drug to establish an evaluation method mainly based on bio-thermodynamics profile detection. Fischer function was used to analyze the chemical and biological data In general, chemical chromatogram can distinguish the expired sample and thermal spectrum of biological activity can distinguish special samples exactly. Thus, we established the evaluation method regarding the quality volatility of Yiqi Fumai freeze-dried powder for injection. The method can be used as a useful supplement in quality control as, and it could provide some technical information for quality control of other varieties of traditional Chinese medicine for injection.