Abstract
To investigate the substrate specificity and regio-selectivity of coumarin glycosyltransferases in transgenic hairy roots of Polygonum multiflorum, esculetin (1) and eight hydroxycoumarins (2-9) were employed as substrates. Nine corresponding glycosides (10-18) involving four new compounds, 6-chloro-4-methylcoumarin 7-O-β-D-glucopyranoside (15), 6-chloro-4-phenylcoumarin 7-O-β-D-glucopyranoside (16), 8-hydroxy-4-methylcoumarin 7-O-β-D-glucopyranoside (17), and 8-allyl-4-methylcoumarin 7-O-β-D-glucopyranoside (18), were biosynthesized by the hairy roots.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Chromatography, High Pressure Liquid
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Coumarins / chemistry
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Coumarins / metabolism*
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Glycosides / biosynthesis*
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Glycosides / chemistry
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Glycosyltransferases / chemistry
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Glycosyltransferases / metabolism*
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Plant Roots / chemistry
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Plant Roots / enzymology*
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Plant Roots / genetics
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Plants, Genetically Modified / chemistry
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Plants, Genetically Modified / enzymology
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Polygonum / chemistry
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Polygonum / enzymology*
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Polygonum / genetics
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Stereoisomerism
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Substrate Specificity
Substances
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Coumarins
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Glycosides
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Glycosyltransferases