Methods for chromatographic removal of endotoxin

Methods Mol Biol. 2012:899:265-75. doi: 10.1007/978-1-61779-921-1_17.

Abstract

Endotoxin removal is critical when producing therapeutic proteins in bacterial systems. This hydrophobic compound can be removed through chromatography or filtration, but presents unique challenges dependent upon protein composition as well as production scale. Here we present a robust method for endotoxin removal at the pilot production scale using fast protein liquid chromatography and buffers specifically engineered for endotoxin removal.

MeSH terms

  • Buffers
  • Cell Wall / chemistry
  • Chromatography, Liquid / methods*
  • Endotoxins / chemistry*
  • Endotoxins / isolation & purification
  • Escherichia coli / chemistry
  • Escherichia coli / genetics
  • Lipopolysaccharides / chemistry*
  • Octoxynol
  • Polyethylene Glycols / chemistry
  • Recombinant Proteins* / biosynthesis
  • Recombinant Proteins* / genetics
  • Recombinant Proteins* / isolation & purification

Substances

  • Buffers
  • Endotoxins
  • Lipopolysaccharides
  • Recombinant Proteins
  • Polyethylene Glycols
  • Octoxynol
  • Nonidet P-40