Ers1 links HP1 to RNAi

Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11258-63. doi: 10.1073/pnas.1204947109. Epub 2012 Jun 25.

Abstract

Pericentromeric heterochromatin formation is mediated by repressive histone H3 lysine 9 methylation (H3K9Me) and its recognition by HP1 proteins. Intriguingly, in many organisms, RNAi is coupled to this process through poorly understood mechanisms. In Schizosaccharomyces pombe, the H3-K9 methyltransferase Clr4 and the heterochromatin protein 1 (HP1) ortholog Swi6 are critical for RNAi, whereas RNAi stimulates H3K9Me. In addition to the endoribonuclease Dcr1, RNAi in S. pombe requires two interacting protein complexes, the RITS complex, which contains an Argonaute subunit, and the RDRC complex, which contains an RNA-dependent RNA polymerase subunit. We previously identified Ers1 (essential for RNAi-dependent silencing) as an orphan protein that genetically acts in the RNAi pathway. Using recombinant proteins, we show here that Ers1 directly and specifically interacts with HP1/Swi6. Two-hybrid assays indicate that Ers1 also directly interacts with several RNAi factors. Consistent with these interactions, Ers1 associates in vivo with the RITS complex, the RDRC complex, and Dcr1, and it promotes interactions between these factors. Ers1, like Swi6, is also required for RNAi complexes to associate with pericentromeric noncoding RNAs. Overexpression of Ers1 results in a dominant-negative phenotype that can be specifically suppressed by increasing levels of the RDRC subunit Hrr1 or of Dcr1, further supporting a functional role for Ers1 in promoting the assembly of the RNAi machinery. Through the interactions described here, Ers1 may promote RNAi by tethering the corresponding enzyme complexes to HP1-coated chromatin, thereby placing them in proximity to the nascent noncoding RNA substrate.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Carrier Proteins / metabolism
  • Chromobox Protein Homolog 5
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Endoribonucleases / metabolism
  • Escherichia coli / metabolism
  • Gene Expression Regulation, Fungal*
  • Gene Silencing
  • Heterochromatin / metabolism
  • Phenotype
  • RNA Interference*
  • Recombinant Proteins / metabolism*
  • Schizosaccharomyces / genetics
  • Schizosaccharomyces / metabolism*
  • Schizosaccharomyces pombe Proteins / metabolism*
  • Two-Hybrid System Techniques

Substances

  • Carrier Proteins
  • Chromosomal Proteins, Non-Histone
  • Ers1 protein, S pombe
  • Heterochromatin
  • Recombinant Proteins
  • Schizosaccharomyces pombe Proteins
  • Swi6 protein, S pombe
  • Tas3 protein, S pombe
  • Chromobox Protein Homolog 5
  • Dcr1 protein, S pombe
  • Endoribonucleases