MicroRNA-23b downregulates peroxiredoxin III in human prostate cancer

FEBS Lett. 2012 Jul 30;586(16):2451-8. doi: 10.1016/j.febslet.2012.06.003. Epub 2012 Jun 16.

Abstract

To investigate the mechanism by which peroxiredoxin III (PRDX3) is altered in human prostate cancer (PCa), we used microRNA (miRNA) target prediction program and miRNA microarray to predict and identify miR-23b as a candidate miRNA that targets PRDX3. We showed that miR-23b suppresses PRDX3 protein expression in human DU145 cells under normal and hypoxic conditions. Additionally, the clinical significance of miR-23b and PRDX3 expression in PCa patients was also confirmed. In conclusion, our data suggest that the effects of PRDX3 in PCa progression may be caused by the regulation function of miR-23b, and consequently, miR-23b may be involved in the response of PCa cells to hypoxia stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Base Sequence
  • Cell Line, Tumor
  • Disease Progression
  • Disease-Free Survival
  • Down-Regulation*
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Hypoxia
  • Male
  • MicroRNAs / metabolism
  • MicroRNAs / physiology*
  • Middle Aged
  • Molecular Sequence Data
  • Oligonucleotide Array Sequence Analysis
  • Peroxiredoxin III / biosynthesis*
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / metabolism*

Substances

  • MIRN23a microRNA, human
  • MicroRNAs
  • PRDX3 protein, human
  • Peroxiredoxin III