Targeting pre-miRNA by peptide nucleic acids: a new strategy to interfere in the miRNA maturation

Artif DNA PNA XNA. 2012 Apr-Jun;3(2):88-96. doi: 10.4161/adna.20911. Epub 2012 Apr 1.

Abstract

PNAs conjugated to carrier peptides have been employed for the targeting of miRNA precursor, with the aim to develop molecules able to interfere in the pre-miRNA processing. The capability of the molecules to bind pre-miRNA has been tested in vitro by fluorescence assayes on Thiazole Orange labeled molecules and in vivo, in K562 cells, evaluating the amount of miRNA produced after treatment of cells with two amounts of PNAs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Down-Regulation / drug effects
  • Humans
  • K562 Cells
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Peptide Nucleic Acids / chemical synthesis
  • Peptide Nucleic Acids / chemistry
  • Peptide Nucleic Acids / metabolism*
  • Peptide Nucleic Acids / pharmacology*
  • Plicamycin / pharmacology

Substances

  • MicroRNAs
  • Peptide Nucleic Acids
  • Plicamycin