Visual detection of DNA mutation using multicolor fluorescent coding

ACS Appl Mater Interfaces. 2012 Jun 27;4(6):2885-90. doi: 10.1021/am300830r. Epub 2012 Jun 18.

Abstract

A visual colorimetric method for detecting multiplex DNA mutations has been developed using multicolor fluorescent coding based on multistep FRET of cationic conjugated polymers. Expensive instruments and technical expertise are not required in this method. Also our visual system provides a quantitative detection by simply analyzing RGB values of images. Genomic DNAs extracted from 60 FFPE colon tissues can be sensitively determined by utilizing our visual assay with a high-throughput manner. Thus, it proves to be sensitive, reliable, cost-effective, simple, and high-throughput for mutation detection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Class I Phosphatidylinositol 3-Kinases
  • DNA / analysis*
  • Fluorenes / chemistry
  • Fluorescein / chemistry
  • Fluorescence Resonance Energy Transfer*
  • Fluorescent Dyes / chemistry*
  • Humans
  • MCF-7 Cells
  • Mutation
  • Phosphatidylinositol 3-Kinases / genetics
  • Phosphatidylinositol 3-Kinases / metabolism
  • Polymers / chemistry
  • Quaternary Ammonium Compounds / chemistry

Substances

  • Fluorenes
  • Fluorescent Dyes
  • Polymers
  • Quaternary Ammonium Compounds
  • poly(9,9-bis(6'-N,N,N-trimethylammonium)hexyl)fluorenephenylene bromide
  • DNA
  • Phosphatidylinositol 3-Kinases
  • Class I Phosphatidylinositol 3-Kinases
  • PIK3CA protein, human
  • Fluorescein