Abstract
An aqueous ethanol extract of Artemisia argyi inhibited the aminoacylation activity of LeuRS from Giardia lamblia (GlLeuRS). The bioassay-guided fractionation of the extract led to the isolation of 3,5-dicaffeoylquinic acid (1), with an IC₅₀ of 5.82 μg/mL. The ester derivatives of 1 were also found to possess strong anti-GlLeuRS effects, with IC₅₀ values of 1.79, 5.51 and 2.56 μg/mL respectively. Anti-giardial assay showed that the derivatives, especially 3,5-dicaffeoylquinic acid propyl ester (4) (IC₅₀=4.62 μg/mL), were effective at killing G. lamblia.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aminoacylation
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Antiprotozoal Agents / isolation & purification
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Antiprotozoal Agents / pharmacology*
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Artemisia / chemistry*
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Chlorogenic Acid / analogs & derivatives*
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Chlorogenic Acid / isolation & purification
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Chlorogenic Acid / pharmacology
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Giardia lamblia / drug effects*
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Giardia lamblia / genetics
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Leucine-tRNA Ligase / antagonists & inhibitors*
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Parasitic Sensitivity Tests
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
Substances
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3,5-dicaffeoylquinic acid propyl ester
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Antiprotozoal Agents
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Plant Extracts
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Chlorogenic Acid
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Leucine-tRNA Ligase
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3,5-dicaffeoylquinic acid