High-resolution melt PCR analysis for rapid identification of Chlamydia abortus live vaccine strain 1B among C. abortus strains and field isolates

J Microbiol Methods. 2012 Sep;90(3):241-4. doi: 10.1016/j.mimet.2012.05.014. Epub 2012 May 28.

Abstract

We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus live vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.

MeSH terms

  • Animals
  • Bacterial Vaccines / genetics*
  • Bacterial Vaccines / immunology
  • Bacterial Vaccines / isolation & purification
  • Cattle
  • Cattle Diseases / immunology
  • Cattle Diseases / microbiology*
  • Cattle Diseases / prevention & control
  • Chlamydia / genetics*
  • Chlamydia / immunology
  • Chlamydia / isolation & purification
  • Chlamydia Infections / immunology
  • Chlamydia Infections / microbiology
  • Chlamydia Infections / prevention & control
  • Chlamydia Infections / veterinary*
  • DNA, Bacterial / genetics
  • Goat Diseases / immunology
  • Goat Diseases / microbiology*
  • Goat Diseases / prevention & control
  • Goats
  • Molecular Typing
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide
  • Sheep
  • Sheep Diseases / immunology
  • Sheep Diseases / microbiology*
  • Sheep Diseases / prevention & control
  • Transition Temperature
  • Vaccines, Live, Unattenuated / genetics
  • Vaccines, Live, Unattenuated / immunology
  • Vaccines, Live, Unattenuated / isolation & purification

Substances

  • Bacterial Vaccines
  • DNA, Bacterial
  • Vaccines, Live, Unattenuated