Sulphation of hirudin in BHK cells

FEBS Lett. 1990 Nov 26;275(1-2):36-8. doi: 10.1016/0014-5793(90)81433-o.

Abstract

Hirudin, a thrombin inhibitor of the leech, was expressed in BHK cells; the alpha 1-antitrypsin signal peptide was used to direct secretion into the culture medium. The recombinant hirudin so produced inhibited thrombin and was shown by labelling experiments with [35S]sulphate to have been posttranslationally modified.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Cricetinae
  • DNA Mutational Analysis
  • Gene Expression
  • Hirudins / metabolism*
  • In Vitro Techniques
  • Kidney
  • Molecular Sequence Data
  • Precipitin Tests
  • Protein Processing, Post-Translational
  • Protein Sorting Signals
  • Recombinant Proteins / metabolism
  • Sulfates / metabolism*
  • Thrombin / antagonists & inhibitors

Substances

  • Hirudins
  • Protein Sorting Signals
  • Recombinant Proteins
  • Sulfates
  • Thrombin