Abstract
Hirudin, a thrombin inhibitor of the leech, was expressed in BHK cells; the alpha 1-antitrypsin signal peptide was used to direct secretion into the culture medium. The recombinant hirudin so produced inhibited thrombin and was shown by labelling experiments with [35S]sulphate to have been posttranslationally modified.
MeSH terms
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Amino Acid Sequence
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Animals
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Cloning, Molecular
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Cricetinae
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DNA Mutational Analysis
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Gene Expression
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Hirudins / metabolism*
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In Vitro Techniques
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Kidney
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Molecular Sequence Data
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Precipitin Tests
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Protein Processing, Post-Translational
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Protein Sorting Signals
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Recombinant Proteins / metabolism
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Sulfates / metabolism*
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Thrombin / antagonists & inhibitors
Substances
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Hirudins
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Protein Sorting Signals
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Recombinant Proteins
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Sulfates
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Thrombin