Analytical evaluation of Sysmex UF-1000i for flow cytometric analysis of peritoneal fluid

Sabrina Buoro; Rosanna Gustinetti; Paola Dominoni; Maria Grazia Alessio; Patrizia Filisetti; Maria Grazia Lucà; Stefano Fagiuoli; Cosimo Ottomano; Giuseppe Lippi

doi:10.1016/j.clinbiochem.2012.05.005

Analytical evaluation of Sysmex UF-1000i for flow cytometric analysis of peritoneal fluid

Clin Biochem. 2012 Oct;45(15):1263-5. doi: 10.1016/j.clinbiochem.2012.05.005. Epub 2012 May 17.

Authors

Sabrina Buoro¹, Rosanna Gustinetti, Paola Dominoni, Maria Grazia Alessio, Patrizia Filisetti, Maria Grazia Lucà, Stefano Fagiuoli, Cosimo Ottomano, Giuseppe Lippi

Affiliation

¹ Laboratorio Analisi Chimico Cliniche, AO Ospedali Riuniti Bergamo, Bergamo, Italy.

PMID: 22609897
DOI: 10.1016/j.clinbiochem.2012.05.005

Abstract

Objective: To evaluate analytical performance of Sysmex UF-1000i for peritoneal fluid analysis.

Methods: Functional sensitivity, imprecision, linearity and comparison studies were performed on peritoneal fluids.

Results: Total imprecision was 1.6-4.7%, functional sensitivity 27/μL for white blood cell (WBC) and 32/μL for total nucleated cell (TNC) count. Linearity was excellent up to 983 cell/μL, carry-over <0.2%, correlation with manual microscopy always greater than 0.992.

Conclusion: The instrument exhibited optimal performance at the conventional WBC diagnostic thresholds.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Ascitic Fluid / cytology*
Cell Count
Flow Cytometry / instrumentation*
Humans
Regression Analysis
Reproducibility of Results
Sensitivity and Specificity