In this study, a rhamnolipid biosurfactant-producing strain, Pseudomonas aeruginosa F-2, was used to recover oil from refinery oily sludge in laboratory and pilot-scale experiments. The optimum values of carbon to nitrogen ratio, temperature, sludge-water ratio and inoculum size for oil recovery were determined as 10, 35 °C, 1:4 and 4%, respectively. An oil recovery of up to 91.5% was obtained with the equipping of draft tubes during the field pilot-scale studies. The results showed that strain F-2 has the potential for industrial applications and may be used in oil recovery from oily sludge.
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