Effects of bushen huoxue yin on brain NF-kB and NO content in the Parkinson's disease model mouse

J Tradit Chin Med. 2012 Mar;32(1):67-70. doi: 10.1016/s0254-6272(12)60034-x.

Abstract

Objective: To observe the effects of Bushen Huoxue Yin on nuclear transcription factor kappa B (NF-KB) and nitric oxide (NO) in the brain of the Parkinson's disease (PD) model mouse.

Methods: Forty-five C57BL/6 mice were randomly divided into three groups; normal, model and BSHXY treatment groups. Concentrations of NF-KB and NO in mouse brain tissue were determined by ELISA and spectrophotometry, respectively.

Results: NF-KB concentration in brain tissue in the model group was 14.04 +/- 4.38 microg x L(-1), which was higher than that in normal (P < 0.01) and BSHXY (P < 0.05) groups. NO content in brain tissue in the model group was 5.93 +/- 0.79 micromol x gprot(-1), which was also higher than that in model (P < 0.01) and BSHXY (P < 0.01) groups. However, there were no significant differences in the content of NF-KB and NO between BSHXY and normal groups (P > 0.05).

Conclusion: The mechanism of BSHXY for treatment of PD is possibly related to inhibition of NF-KB activation and decreased NO content in the brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / drug effects
  • Brain / metabolism*
  • Disease Models, Animal
  • Drugs, Chinese Herbal / administration & dosage*
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • NF-kappa B / metabolism*
  • Nitric Oxide / metabolism*
  • Parkinson Disease / drug therapy*
  • Parkinson Disease / metabolism

Substances

  • Drugs, Chinese Herbal
  • NF-kappa B
  • bushen huoxue
  • Nitric Oxide