Aqueous microwave-assisted solid-phase peptide synthesis using Fmoc strategy: in-water synthesis of "difficult sequences"

Keiko Hojo; Hideki Ichikawa; Asaki Hara; Mare Onishi; Koichi Kawasaki; Yoshinobu Fukumori

doi:10.2174/092986612803217114

Aqueous microwave-assisted solid-phase peptide synthesis using Fmoc strategy: in-water synthesis of "difficult sequences"

Protein Pept Lett. 2012 Nov;19(11):1231-6. doi: 10.2174/092986612803217114.

Authors

Keiko Hojo¹, Hideki Ichikawa, Asaki Hara, Mare Onishi, Koichi Kawasaki, Yoshinobu Fukumori

Affiliation

¹ Faculty of Pharmaceutical Sciences & Cooperative Research Center of Life Sciences, Kobe Gakuin University, Chou-ku, Kobe, Japan. hojo@pharm.kobegakuin.ac.jp

PMID: 22587785
DOI: 10.2174/092986612803217114

Abstract

A microwave assisted peptide synthesis in water using nanosized Fmoc-amino acids was developed. 5, 7, and 10 mer peptides (Leu-enkephalinamide, dermorphinamide, and a typical difficult sequence, ACP (65-74) peptide) were successfully synthesized in water according to Fmoc chemistry using water-dispersible nanoparticles with microwave irradiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amino Acids / chemistry*
Fluorenes / chemistry*
Microwaves*
Nanoparticles / chemistry
Particle Size
Peptides / chemical synthesis*
Peptides / chemistry
Solid-Phase Synthesis Techniques / methods*
Water / chemistry*

Substances

Amino Acids
Fluorenes
N(alpha)-fluorenylmethyloxycarbonylamino acids
Peptides
Water