Detection and characterization of hepatitis E virus in domestic pigs of different ages in Portugal

Zoonoses Public Health. 2012 Nov;59(7):477-81. doi: 10.1111/j.1863-2378.2012.01488.x. Epub 2012 May 14.

Abstract

This study represents the primary hepatitis E virus (HEV) surveillance in domestic pigs in Portugal, five pig farms were investigated in 5 different Portuguese regions, ten faecal samples were collected at four different stages of the production. All faecal samples were tested for hepatitis E virus by real-time RT-PCR. At least one sample from each farms of all age groups tested positive for HEV. The prevalence in the pig herds varied from 10% to 30% and the mean prevalence was 32% in weaners, 20% in growers, 32% in fatteners and 4% in adult dry sows. Phylogenetic analysis of the detected HEV sequences indicated that the circulating virus strains belong under the genotype 3.

MeSH terms

  • Age Distribution
  • Animals
  • Feces / virology
  • Female
  • Genotype
  • Hepatitis E / epidemiology
  • Hepatitis E / genetics
  • Hepatitis E / veterinary*
  • Hepatitis E / virology
  • Hepatitis E virus / classification
  • Hepatitis E virus / genetics
  • Hepatitis E virus / isolation & purification*
  • Male
  • Phylogeny
  • Portugal / epidemiology
  • Prevalence
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sus scrofa
  • Swine
  • Swine Diseases / epidemiology*
  • Swine Diseases / genetics
  • Swine Diseases / virology
  • Zoonoses

Substances

  • RNA, Viral