Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale

Chem Commun (Camb). 2012 Jun 18;48(48):6001-3. doi: 10.1039/c2cc17878h. Epub 2012 May 10.

Abstract

A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Enzymes
  • Escherichia coli / chemistry*
  • Escherichia coli / metabolism*
  • Flavins / chemistry*
  • Flavins / metabolism*
  • Humans
  • Mixed Function Oxygenases / chemistry*
  • Mixed Function Oxygenases / metabolism
  • Moclobemide / chemical synthesis*
  • Moclobemide / chemistry

Substances

  • Enzymes
  • Flavins
  • Mixed Function Oxygenases
  • Moclobemide