Abstract
A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Enzymes
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Escherichia coli / chemistry*
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Escherichia coli / metabolism*
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Flavins / chemistry*
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Flavins / metabolism*
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Humans
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Mixed Function Oxygenases / chemistry*
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Mixed Function Oxygenases / metabolism
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Moclobemide / chemical synthesis*
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Moclobemide / chemistry
Substances
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Enzymes
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Flavins
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Mixed Function Oxygenases
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Moclobemide