The aqueous and successive extracts of the fruit pulp of Emblica officinalis and fresh leaves and stems of Ocimum sanctum were prepared and evaluated for antimicrobial activity. The successive extracts such as petroleum ether,chloroform, ethyl acetate and methanol were prepared by successive solvent extraction method and aqueous extract by maceration process and screened for antimicrobial activity against gram positive bacteria Staphylococcus aureus, gram negative bacteria E.coli and fungal strains of Candida species by using agar cup plate method. The extracts showed different degree of activity against pathogenic microbes. The results obtained were compared with standard drugs Amoxicillin (10μg) and Amphotericin B(10μg). The methanolic extract of Emblica officinalis was found to be more effective than the leaf and stem extracts of Ocimum sanctum in inhibiting all the microbial strains.