5-hydroxytryptamine level and 5-HT2A receptor mRNA expression in the guinea pigs eyes with spectacle lens-induced myopia

Ji-Wen Yang; Yan-Chun Xu; Lin Sun; Xiao-Dan Tian

doi:10.3980/j.issn.2222-3959.2010.04.05

5-hydroxytryptamine level and 5-HT2A receptor mRNA expression in the guinea pigs eyes with spectacle lens-induced myopia

Int J Ophthalmol. 2010;3(4):299-303. doi: 10.3980/j.issn.2222-3959.2010.04.05. Epub 2010 Dec 18.

Authors

Ji-Wen Yang¹, Yan-Chun Xu, Lin Sun, Xiao-Dan Tian

Affiliation

¹ Department of Ophthalmology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.

PMID: 22553578
PMCID: PMC3340756
DOI: 10.3980/j.issn.2222-3959.2010.04.05

Abstract

Aim: To investigate 5-hydroxytryptamine (5-HT) function and 5-HT receptor 2A (5-HT2A) mRNA expression in the formation of lens-induced myopia (LIM).

Methods: Lens-induced myopia construction method was applied to generate myopia on guinea pig right eye (LIM eye).

Results: LIM eyes formed significant myopia with longer axial length. 5-HT level in retina, choroids and sclera from LIM eyes was significantly higher than that in control group. 5-HT2A mRNA expression was also significantly up-regulated.

Conclusion: Refraction lens could induce myopia in guinea pig and 5-HT may play an important role in the formation of myopia by binding with 5-HT2A receptor.

Keywords: 5-HT receptor 2A; 5-hydroxytryptamine; guinea pig; high performance liquid chromatography/electrochemical detection; lens-induced myopia.