Automated capillary electrophoresis with on-line preconcentration by solid phase extraction using a sequential injection manifold and contactless conductivity detection

Thanh Duc Mai; Benjamin Bomastyk; Hong Anh Duong; Hung Viet Pham; Peter C Hauser

doi:10.1016/j.aca.2012.03.035

Automated capillary electrophoresis with on-line preconcentration by solid phase extraction using a sequential injection manifold and contactless conductivity detection

Anal Chim Acta. 2012 May 21:727:1-7. doi: 10.1016/j.aca.2012.03.035. Epub 2012 Apr 1.

Authors

Thanh Duc Mai¹, Benjamin Bomastyk, Hong Anh Duong, Hung Viet Pham, Peter C Hauser

Affiliation

¹ University of Basel, Department of Chemistry, Spitalstrasse 51, 4056 Basel, Switzerland.

PMID: 22541815
DOI: 10.1016/j.aca.2012.03.035

Abstract

An extension of a capillary electrophoresis instrument coupled to a sequential injection analysis manifold was developed for automated measurements with on-line solid-phase extraction preconcentration. An in-house built capacitively coupled contactless conductivity detector was employed for sensitive detection with narrow capillaries of 25 μm internal diameter. The system was assembled into standardized 19 in. frames and racks for easy transport and mobile deployment. The system can be left running unattendedly without manual intervention with good operation stability. To demonstrate the application of the system, a method for the determination of four drugs, namely ibuprofen, diclofenac, naproxen and bezafibrate, was developed with enrichment factors of up to several hundreds. Detection of the drug residues down to the nM-scale was found possible and the method was found suitable for the detection of ibuprofen in the waste water of a hospital in Hanoi.