A rapid mismatch polymerase chain reaction assay to detect carriers of complex vertebral malformation in Holstein cattle

J Vet Diagn Invest. 2012 May;24(3):568-71. doi: 10.1177/1040638712441605.

Abstract

Three Holstein sires were identified as complex vertebral malformation (CVM) carriers using the polymerase chain reaction-primer introduced restriction analysis (PCR-PIRA) method. Using the carriers as positive controls, the PCR mismatch amplification mutation assay (MAMA PCR) method was developed and validated by sequence analysis. With MAMA PCR, 154 Chinese Holstein sires were tested for CVM, among which 24 were confirmed to be CVM carriers. With DNA isolated from hair follicles, 10 daughters of one CVM-positive bull were detected, among which 7 were confirmed to be CVM carriers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cattle Diseases / genetics*
  • Cattle Diseases / pathology
  • Cervical Vertebrae / abnormalities*
  • DNA / chemistry
  • DNA / genetics
  • Female
  • Genetic Carrier Screening / methods*
  • Male
  • Point Mutation
  • Real-Time Polymerase Chain Reaction / methods
  • Real-Time Polymerase Chain Reaction / veterinary*
  • Spinal Diseases / genetics
  • Spinal Diseases / pathology
  • Spinal Diseases / veterinary*

Substances

  • DNA