Abstract
We recently observed that lipoteichoic acid (LTA) isolated from Lactobacillus plantarum inhibited endotoxin-mediated inflammation of the immune cells and septic shock in a mouse model. Here, we examined the inhibitory role of L. plantarum LTA (pLTA) on the inflammatory responses of intestinal epithelial cells (IEC). The human colon cell line, HT-29, increased interleukin (IL)-8 expression in response to recombinant human tumor necrosis factor (TNF)-alpha, but not in response to bacterial ligands and interferon (IFN)-gamma. TNF-α also increased the production of inducible nitric oxide synthase (iNOS), nitric oxide (NO), and intercellular adhesion molecule 1 (ICAM-1) through activation of p38 mitogen-activated protein kinase (MAPK) from HT-29 cells. However, the inflammatory response of HT-29 on TNF-α stimulation was significantly inhibited by pLTA treatment. This pLTA-mediated inhibition accompanied the inhibition of nuclear factor (NF)-kappa B and MAPKs. Our data suggest that pLTA regulates cytokine-mediated immune responses and may be a good candidate for maintaining intestinal homeostasis against excessive inflammation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Adhesion / drug effects
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Cell Line, Tumor
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Epithelial Cells / drug effects
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Epithelial Cells / metabolism
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Epithelial Cells / pathology
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HT29 Cells
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Humans
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Inflammation / drug therapy*
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Inflammation / metabolism
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Intercellular Adhesion Molecule-1 / metabolism
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Interferon-gamma / metabolism
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Interleukin-8 / antagonists & inhibitors
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Interleukin-8 / metabolism
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Intestinal Mucosa / drug effects*
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Intestinal Mucosa / metabolism
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Intestinal Mucosa / pathology
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Intestines / drug effects*
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Intestines / pathology
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Lactobacillus plantarum / chemistry*
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Lactobacillus plantarum / metabolism
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Lipopolysaccharides / pharmacology*
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NF-kappa B / antagonists & inhibitors
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NF-kappa B / metabolism
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Nitric Oxide / antagonists & inhibitors
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Nitric Oxide / metabolism
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Nitric Oxide Synthase Type II / metabolism
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Teichoic Acids / pharmacology*
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Tumor Necrosis Factor-alpha / metabolism*
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p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors
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p38 Mitogen-Activated Protein Kinases / metabolism
Substances
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Interleukin-8
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Lipopolysaccharides
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NF-kappa B
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Teichoic Acids
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Tumor Necrosis Factor-alpha
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Intercellular Adhesion Molecule-1
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Nitric Oxide
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lipoteichoic acid
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Interferon-gamma
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Nitric Oxide Synthase Type II
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p38 Mitogen-Activated Protein Kinases