Graphene-oxide-based immunosensing through fluorescence quenching by peroxidase-catalyzed polymerization

Seong Yoon Lim; Junhyoung Ahn; Joon Seok Lee; Min-Gon Kim; Chan Beum Park

doi:10.1002/smll.201200379

Graphene-oxide-based immunosensing through fluorescence quenching by peroxidase-catalyzed polymerization

Small. 2012 Jul 9;8(13):1994-9. doi: 10.1002/smll.201200379. Epub 2012 Apr 17.

Authors

Seong Yoon Lim¹, Junhyoung Ahn, Joon Seok Lee, Min-Gon Kim, Chan Beum Park

Affiliation

¹ Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, 335 Science Road, Daejeon, Korea.

PMID: 22511548
DOI: 10.1002/smll.201200379

Abstract

A graphene oxide (GO)-based immunosensor is developed for the detection of interleukin-5 (IL-5), a key cytokine associated with asthma pathology and eosinophilia. The immunosensing platform utilizes the innate fluorescence of GO, not demanding biomolecules labeled with fluorescent dyes. The GO-based immunoassay exhibits high specificity for IL-5 among other cytokines and is not affected by nonspecific proteins in human serum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / methods*
Graphite / chemistry*
Nanotechnology / methods*
Peroxidase / metabolism*
Polymerization

Substances

Graphite
Peroxidase