Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad

Appl Environ Microbiol. 2012 Jun;78(12):4493-6. doi: 10.1128/AEM.00715-12. Epub 2012 Apr 6.

Abstract

We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenase-encoding genes.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Bacteriological Techniques / methods
  • Biotransformation*
  • Dioxins / metabolism*
  • Genes, Bacterial
  • Metabolic Networks and Pathways / genetics
  • Plasmids*
  • Real-Time Polymerase Chain Reaction / methods*
  • Soil Microbiology*
  • Sphingomonas / genetics*
  • Sphingomonas / growth & development*
  • Sphingomonas / isolation & purification
  • Sphingomonas / metabolism

Substances

  • Dioxins
  • dibenzo(1,4)dioxin