Abstract
We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenase-encoding genes.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
MeSH terms
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Bacteriological Techniques / methods
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Biotransformation*
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Dioxins / metabolism*
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Genes, Bacterial
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Metabolic Networks and Pathways / genetics
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Plasmids*
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Real-Time Polymerase Chain Reaction / methods*
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Soil Microbiology*
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Sphingomonas / genetics*
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Sphingomonas / growth & development*
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Sphingomonas / isolation & purification
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Sphingomonas / metabolism
Substances
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Dioxins
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dibenzo(1,4)dioxin