Abstract
On high performance liquid chromatography, the caffeoylquinic acid (CQ) occupying the highest proportion of the water-ethanol (7:3) extract of Aster glehni (Compositae) leaves was 3-Op-coumaroylquinic acid (46.10 ± 4.22 mg/g of dried weight) among CQs tested. The IC₅₀ of the water-ethanol (7:3) extract was 4.23 ± 0.24 μg/mL in the peroxynitrite (ONOO(-))-scavenging assay. Phytochemical isolation from A. glehni extract yielded three kaempferol glycosides. The water-ethanol (7:3) extract and both p-coumaric acid and caffeic acid, phenylpropanoid moieties of CQs, had sedative effects in pentobarbital-induced sleeping time in mice and anticonvulsant effects in pentylenetetrazole-induced mice. Furthermore, the phenolic substance-rich W-E (7:3) extract of A. glehni could be used to treat anxiety or convulsion partly due to its peroxynitrite-scavenging mechanism.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anticonvulsants / chemistry
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Anticonvulsants / isolation & purification
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Anticonvulsants / pharmacology*
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Aster Plant / chemistry
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Chromatography, High Pressure Liquid
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Disease Models, Animal
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Ethanol / chemistry
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Free Radical Scavengers / chemistry
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Free Radical Scavengers / isolation & purification
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Free Radical Scavengers / pharmacology*
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Hypnotics and Sedatives / chemistry
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Hypnotics and Sedatives / isolation & purification
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Hypnotics and Sedatives / pharmacology*
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Kaempferols / isolation & purification
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Kaempferols / pharmacology
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Male
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Mice
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Mice, Inbred ICR
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Pentobarbital / pharmacology
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Pentylenetetrazole
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Peroxynitrous Acid / chemistry
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Phenols / chemistry
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Phenols / isolation & purification
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Phenols / pharmacology*
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Plant Leaves
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Quinic Acid / analogs & derivatives
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Quinic Acid / isolation & purification
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Quinic Acid / pharmacology
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Seizures / chemically induced
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Seizures / prevention & control*
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Sleep / drug effects*
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Solvents / chemistry
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Time Factors
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Water / chemistry
Substances
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Anticonvulsants
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Free Radical Scavengers
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Hypnotics and Sedatives
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Kaempferols
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Phenols
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Plant Extracts
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Solvents
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caffeoylquinic acid
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Quinic Acid
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Water
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Peroxynitrous Acid
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Ethanol
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Pentobarbital
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Pentylenetetrazole