The aim of this study was to experimentally evaluate temperature monitoring with a Fluoroptic® temperature probe in the presence of laser irradiation from a Nd:YAG laser, which is mainly used for clinical MR-guided laser-induced interstitial thermotherapy. Temperature measurements were performed using a Fluoroptic® probe in comparison to a thermocouple probe in a gel phantom and an ex vivo pig liver at distances of 6.5 to 14 mm to the laser applicator (laser energy of 30.8 W). To evaluate the artifacts in the temperature measurement, the laser was turned on and off three times during the entire experiment. A comparison of the fiber-optic measurements with MR thermometry was also performed in pig liver by means of the proton resonance frequency method at a distance of 6 mm. Depending on the distance, the temperature measured by the fiber-optic probe deviated from the thermocouple probe temperature. The phantom deviations of 0.4 to 34.3 % were observed. The differences in the liver were smaller and ranged from 1.6 to 5.2 %. The Bland-Altman mean of differences between MR and fiber-optic temperature measurements was 0.02 °C and the 95 % limits of agreement value was ± 2.25°C. During laser application, considerable artifacts occurred in the Fluoroptic® measurements in short distances which was induced by laser energy absorption by the probe coating. No artifacts were verifiable at a distance of 14 mm in both mediums. The good conformity with MR thermometry resulted from the shorter turn-on times of the laser since the laser irradiation had only a minor effect on the measurements.