2D gels and their third-dimension potential

Jorge B Schvartzman; María-Luisa Martínez-Robles; Virginia López; Pablo Hernández; Dora B Krimer

doi:10.1016/j.ymeth.2012.03.013

2D gels and their third-dimension potential

Methods. 2012 Jun;57(2):170-8. doi: 10.1016/j.ymeth.2012.03.013. Epub 2012 Mar 24.

Authors

Jorge B Schvartzman¹, María-Luisa Martínez-Robles, Virginia López, Pablo Hernández, Dora B Krimer

Affiliation

¹ Department of Cell Proliferation and Development, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain. schvartzman@cib.csic.es

PMID: 22465282
DOI: 10.1016/j.ymeth.2012.03.013

Abstract

Two-dimensional (2D) agarose gel electrophoresis is one of the most powerful methods to analyze the mass and shape of replication intermediates. It is often use to map replication origins but it is also useful to characterize termination of replication, replication fork barriers and even replication fork reversal. Here, we present protocols, figures and movies with a thorough description of different modes of replication for linear DNA fragments and the corresponding patterns they generate in 2D gels.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Autoradiography
DNA Replication*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics*
DNA, Bacterial / isolation & purification
DNA, Circular / chemistry
DNA, Circular / genetics*
DNA, Circular / isolation & purification
Electrophoresis, Agar Gel / methods
Electrophoresis, Gel, Two-Dimensional / methods
Microscopy, Atomic Force
Models, Molecular
Nucleic Acid Conformation
Plasmids / chemistry
Plasmids / genetics*
Plasmids / isolation & purification

Substances

DNA, Bacterial
DNA, Circular