Serologic, biologic, and western blot analysis of a T suppressor factor with specificity for the hapten 4-hydroxy-3-nitrophenyl acetyl derived from serum-free medium

J Immunol. 1990 Dec 1;145(11):3570-7.

Abstract

A T cell hybridoma producing a T suppressor factor (TsF) with specificity for the hapten nitrophenyl was converted to long term growth in serum-free medium and its product tested by serology, bioactivity, and Western blot analysis. Results indicated that Ag-specific suppressive activity was present in serum-free medium and this TsF could exhibit the characteristics ascribed to it by various groups: it could bind nominal Ag with specificity, it was bound by anti-TsF mAb, and it could mediate Ag-specific suppression both in vivo and in vitro. Western blot and SDS-PAGE analysis of this purified TsF revealed a 43-kDa single chain protein.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Western
  • Culture Media
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Haptens / immunology*
  • Hybridomas / chemistry*
  • Hypersensitivity, Delayed
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred CBA
  • Nitrophenols / immunology*
  • Phenylacetates
  • Suppressor Factors, Immunologic / analysis*
  • Suppressor Factors, Immunologic / immunology

Substances

  • Culture Media
  • Haptens
  • Nitrophenols
  • Phenylacetates
  • Suppressor Factors, Immunologic
  • 4-hydroxy-5-nitrophenyl acetic acid