RUNX3 mediates suppression of tumor growth and metastasis of human CCRCC by regulating cyclin related proteins and TIMP-1

PLoS One. 2012;7(3):e32961. doi: 10.1371/journal.pone.0032961. Epub 2012 Mar 22.

Abstract

Here we presented that the expression of RUNX3 was significantly decreased in 75 cases of clear cell renal cell carcinoma (CCRCC) tissues (p<0.05). Enforced RUNX3 expression mediated 786-O cells to exhibit inhibition of growth, G1 cell-cycle arrest and metastasis in vitro, and to lost tumorigenicity in nude mouse model in vivo. RUNX3-induced growth suppression was found partially to regulate various proteins, including inhibition of cyclinD1, cyclinE, cdk2, cdk4 and p-Rb, but increase of p27(Kip1), Rb and TIMP-1. Therefore, RUNX3 had the function of inhibiting the proliferative and metastatic abilities of CCRCC cells by regulating cyclins and TIMP1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Carcinoma, Renal Cell / metabolism
  • Carcinoma, Renal Cell / pathology*
  • Cell Division / physiology
  • Core Binding Factor Alpha 3 Subunit / physiology*
  • Cyclins / physiology*
  • Flow Cytometry
  • Humans
  • Kidney Neoplasms / metabolism
  • Kidney Neoplasms / pathology*
  • Mice
  • Mice, Nude
  • Neoplasm Metastasis*
  • Real-Time Polymerase Chain Reaction
  • Tissue Inhibitor of Metalloproteinase-1 / physiology*

Substances

  • Core Binding Factor Alpha 3 Subunit
  • Cyclins
  • Runx3 protein, human
  • TIMP1 protein, human
  • Tissue Inhibitor of Metalloproteinase-1