In an infected wound, bacteria are present not only on the surface but also within the granulation tissue. The presence of bacteria inside the granulation tissue is one of the reasons for our inability to control infection. We have developed an in vitro model of an infected wound using Luria-Bertani (LB) agar media and non-pathogenic Escherichia coli, and compared the differences in the antibacterial effects of various types of antibacterial wound dressings. The results have shown that the penetrating antibacterial effects differed according to the type of wound dressing selected. As the thickness of the layer of the LB agar media increased, the potency of antibacterial effects caused by penetration decreased. In conclusion, it was suggested that antibacterial wound dressings can have antibacterial effects against bacteria not only on the surface of an infected wound but inside, so it is necessary to use different wound dressings according to the conditions of each infected wound.