Abstract
Administration of short-chain fructooligosaccharide (scFOS) is known to lower serum triglyceride levels in rats fed a high-fat diet, but the molecular mechanisms remain unclear. This study aimed to identify marker genes for lipid-lowering effect of scFOS administration. The changes in hepatic gene expressions in rats fed scFOS were investigated using DNA microarray and quantitative RT-PCR analysis. The DNA microarray showed that phytanoyl-CoA 2-hydroxylase 2 (Phyh2), lipoprotein lipase (Lpl) and tyrosine aminotransferase (Tat) were significantly affected by scFOS administration (p < .05). Since Lpl is involved in lipid metabolism, the up-regulation of Lpl in the liver can be a potential marker of the lipid-lowering effect of scFOS.
MeSH terms
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Animals
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Coenzyme A / metabolism
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DNA / analysis
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Enzyme Inhibitors / pharmacology*
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Enzymes / genetics*
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Enzymes / metabolism
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Fructose / pharmacology*
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Gene Expression / drug effects
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Genetic Markers
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Hypolipidemic Agents / pharmacology*
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Lipid Metabolism / drug effects
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Lipoprotein Lipase / genetics
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Lipoprotein Lipase / metabolism
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Liver / drug effects*
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Liver / metabolism
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Male
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Microarray Analysis
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Mixed Function Oxygenases / genetics
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Mixed Function Oxygenases / metabolism
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Oligosaccharides / pharmacology*
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Phytanic Acid / analogs & derivatives
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Phytanic Acid / metabolism
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Rats, Wistar
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Reverse Transcriptase Polymerase Chain Reaction
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Triglycerides / blood
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Triglycerides / genetics*
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Triglycerides / metabolism
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Tyrosine Transaminase / genetics
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Tyrosine Transaminase / metabolism
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Up-Regulation
Substances
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Enzyme Inhibitors
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Enzymes
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Genetic Markers
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Hypolipidemic Agents
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Oligosaccharides
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Triglycerides
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phytanoyl-coenzyme A
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Phytanic Acid
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Fructose
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DNA
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Mixed Function Oxygenases
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Phyh protein, rat
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Tyrosine Transaminase
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Lipoprotein Lipase
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Coenzyme A